Relationship between the expression of cyclin-dependent kinase 2,7,9 in prostate cancer tissues and clinicopathological features as well as prognosis

Objective: To study the relationship between the expression of cyclin-dependent kinase(CDK) 2,CDK7, CDK9 in prostate cancer(PC) tissues and clinicopathological features as well as prognosis. Methods: A total of 167 PC patients admitted to Harbin 242 Hospital from March 2016 to March 2018 were selected, and cancer tissues and adjacent normal prostate tissues were collected during surgery. Immunohistochemical staining was used to detect the expression of CDK2, CDK7, and CDK9 proteins, and to analyze the relationship between CDK2, CDK7, CDK9 and clinical pathological characteristics of PC. Survival data of patients were collected during a 5-year follow-up, Kaplan-Meier was used to analyze the survival rates of CDK2, CDK7, CDK9 negative and positive expression patients, and univariate and multivariate Cox regression was used to analyze the factors influencing the prognosis of PC patients. Results: Compared with adjacent normal prostate tissue, the positive expression rates of CDK2, CDK7, and CDK9 in PC tissues were increased(P<0.05). The lymph node metastasis,elevated preoperative free PSA content, higher Gleaso score, and advanced TNM stage were associated with increased positive expression rates of CDK2, CDK7, and CDK9(P<0.05). The Kplan Meier analysis results showed that the survival rates of CDK2, CDK7, and CDK9 positive expression groups were lower than those of the negative expression groups(P<0.05). The univariate Cox regression analysis showed that preoperative free PSA, Gleaso score, lymph node metastasis, TNM staging, and expression of CDK2, CDK7, and CDK9 were correlated with the prognosis of PC patients(P<0.05). The multivariate Cox analysis results showed that preoperative free PSA≥1.9 ng/mL, Gleaso score≥7 points, lymph node metastasis, TNM stage Ⅲ, CDK2 positive expression, CDK7 positive expression, and CDK9 positive expression were risk factors for poor prognosis of PC(P<0.05). Conclusion: CDK2, CDK7 and CDK9 are highly expressed in PC tissues, and their expression levels are correlated with preoperative free PSA, Gleaso score, lymph node metastasis, TNM stage and prognosis of PC patients.