Study on the quality standard of Yigan tablet

Objective: To improve the quality standard of Yigan tablet.Methods: Isatidis Radix and Polygonum cuspidatum in Yigan tablet were identified with thin layer chromatography (TLC).The content of (R, S)-Goitrin and Emodin in Yigan tablet was determined by high performance liquid chromatography (HPLC).Results: The spots of Isatidis Radix and Polygonum cuspidatum were clear, well separated and negative without interference when identified.The peak area of(R, S)-Goitrin ranged from 0.03 μg to 0.15 μg had perfect linear relationship(r=0.9998) with the injection volume, and the average recovery rate was 97.05%; the peak area of Emodin ranged from 0.0495 μg to 0.297 μg had perfect linear relationship (r=1.0000) with the injection volume, and the average recovery rate was 99.24%.Conclusion: The quality standard can not only effectively identify Isatidis Radix and Polygonum cuspidatum but also can effectively determine the content of (R, S)-Goitrin and Emodin.The method is simple, reliable and repeatable, and can be used for the quality control of Yigan tablet.