Correlation between ANAPC1 expression level in peripheral blood leukocytes and coronary heart disease and its diagnostic value

Objective: To investigate the correlation between anaphase-promoting complex subunit 1(ANAPC1)in peripheral blood leukocytes (PBLs) and coronary heart disease (CHD) and its diagnostic value.Methods: Gene expression datasets and single-cell RNA sequencing datasets were retrieved from the Gene Expression Omnibus (GEO) database to assess the expression of ANAPC1 in PBLs and other cells of CHD patients.Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect and compare the expression level of ANAPC1 in 70 CHD patients and control samples.Receiver operating characteristic (ROC) curve and summary ROC (SROC)curve were used to evaluate the diagnostic value of ANAPC1 in CHD.The CDB database was used to search for potential upstream transcription factors of ANAPC1.ANAPC1 differentially co-expressed genes were subjected to enrichment analysis using gene ontology (GO), disease ontology (DO), and the Kyoto Encyclopedia of Genes and Genomes(KEGG).Results: GEO database analysis showed that the expression level of ANAPC1 in PBLs in CHD patients was decreased (total SMD=-0.74, 95% CI:-1.36 to-0.13), the area under SROC curve was 0.76, the sensitivity was 0.73, and the specificity was 0.67.RT-qPCR of clinical samples showed that the expression level of ANAPC1 in PBLs of CHD patients was decreased, the area under ROC curve was 0.71, the sensitivity was 0.80, and the specificity was 0.61.A search for datasets related to single-cell RNA sequencing in the GEO database revealed that the expression levels of ANAPC1 in different cells at different periods were not significantly different.The prediction of upstream potential transcription factors showed that the expression of ANAPC1 was positively regulated by ETS1 (SMD=-0.53, 95% CI:-0.99 to-0.06) and GATA3 (SMD=-0.46, 95% CI:-0.87 to-0.06)and negatively regulated by IRAK1(SMD=0.42, 95%CI:0.07 to 0.77)and ERF(SMD=0.27, 95%CI:0.03 to 0.51).DO enrichment analysis suggested that ANAPC1 differentially negatively co-expressed genes were associated with diseases such as atherosclerosis and atherosclerotic heart disease.Conclusion: The downregulation of ANAPC1 expression in PBLs in CHD patients is positively correlated with the occurrence and development of CHD, and has certain diagnostic value for CHD.