Effect of exercise on blood glucose in type 2 diabetes mellitus mice based on gut microbiota

Objective: To investigate the effect of exercise on blood glucose, body weight and glucagon-like pep-tide-1(GLP-1)in type 2 diabetes mellitus(T2DM)mice based on gut microbiota.Methods: Six 8-week-old male C57BKS mice were used as wild-type control group (DZ), and eighteen 8-week-old male db/db mice were used as T2DM mouse models and randomly divided into T2DM model+combined exercise group (F group), T2DM model+enema group (SY group), and T2DM model group (TJ group), with 6 mice in each group.The F group underwent aerobic and resistance exercise for 8 weeks.In the SY group, the feces of mice in the F group after 8 weeks of exercise were made into suspension enema, twice a day for 14 days.In the TJ group, the feces suspen-sion made from DZ mice was given enema twice a day for 14 days.No intervention was performed in the DZ group.After the end of enema, the fecal gut microbiota, blood glucose, body weight and serum GLP-1 content were detected.Results: The chao 1 index and shannon index of gut microbiota in the SY group were significantly higher than those in the DZ and TJ groups(P< 0.05); at the phylum level, compared with the DZ group, the rela-tive abundance of Firmicutes in the SY group increased (P< 0.05), while the relative abundance ratios of Proteo-bacteria and Bacteroidetes decreased(P< 0.05); at the genus level, compared with the DZ group, the SY group in-creased the relative abundance of Lachnospiraceae(P< 0.05).Compared with the TJ group, the proportion of Fir-micutes increased, and the proportion of Proteobacteria decreased in the SY group (both P< 0.05).The level of GLP-1 in the SY group was higher than that in the DZ and TJ groups, and the level of blood glucose and body weight were lower than those in the DZ and TJ groups, and the differences were statistically signifi-cant (P< 0.05).Conclusion: Combined aerobic-resistance exercise can improve the abundance and structure of beneficial bacteria in gut microbiota, increase the intestinal short-chain fatty acid producing bacteria and serum GLP-1 level, and reduce blood glucose and body weight in T2DM mice, thereby improving T2DM.