Metabonomics study of atorvastatin combined with Zhentongji capsule on hyperlipidemia model of rats

Objective: To explore the therapeutic effect and mechanism of atorvastatin combined with Zhenton-gji capsule on hyperlipidemia model rats through metabonomics.Methods: Six SD rats were randomly selected as the control group, and they were given normal diet and water.The other animals were given high-fat feed, and after 4 weeks, triglyceride(TG)and cholesterol(CHO)increased significantly, which was regarded as the success of the model.According to CHO index, they were divided into model group, western medicine group (atorvas-tatin calcium tablet, 0.9 mg/kg), traditional Chinese medicine group(Zhentongji capsule, 0.216 g/kg)and integrat-ed traditional Chinese and western medicine group (both combined).After 8 weeks of continuous administration, the serum lipid levels of animals in each group were detected, and the liver tissue was used for high-resolution un-targeted metabolomics research.Results: The levels of TG, CHO, high density lipoprotein (HDL)and low densi-ty lipoprotein (LDL) in the model group were significantly higher than those in the control group (P< 0.05).Compared with the model group, the blood lipid lev-els in the western medicine group, the traditional Chinese medicine group and the integrated tradition-al Chinese and western medicine group significantly reduced (P< 0.05).Compared with the control group, 138 different metabolites were detected in the model group.Compared with the model group, 64 different metabolites were detected in the western medicine group, 123 in the traditional Chinese medicine group and 106 in the integrated traditional Chinese and western medicine group.Compared with the western med-icine group, 101 different metabolites were detected in the traditional Chinese medicine group.Through correla-tion analysis and KEGG pathway analysis, it was mainly involved aging, lipid metabolism, amino acid metabo-lism, membrane transport and other pathways.Conclusion: Atorvastatin combined with Zhentongji capsule can effectively improve the blood lipid levels of hyperlipidemia rats.Metabolomics analysis shows that it may achieve the purpose of inhibiting liver cell necrosis by regulating the expression of related factors in cGMP-PKG signaling pathway.