LncRNA HOTAIR activating SHH signaling pathway by targeting miR-126 to promote the occurrence and development of colorectal cancer

Objective:To investigate the targeting relationship between miR-126 and lncRNA HOTAIR and its regulatory effect on SHH signaling pathway, and to study the molecular mechanism in the occurrence and development of colorectal cancer.Methods:Samples of normal colorectal tissues, colorectal polyps, atypical hyperplasia, precancerous lesions, colorectal cancer tissues and adjacent normal tissues were obtained from patients who underwent surgery in Cangzhou People’s Hospital of Hebei Province from February 2018 to February 2019.Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry were used to analyze the expression of HOTAIR, miR-126 and SHH.StarBase, luciferase assay, RNA co-immunoprecipitation and RT-qPCR were used to verify the relationship of miR-126 with HOTAIR and SHH.MTT, colony-forming assay, scratch assay and Transwell test were performed to detect cell viability, proliferation, migration and invasion.Results:The expression of HOTAIR and SHH, Glil, Smo proteins in colorectal cancer tissues were significantly up-regulated, and the expression of miR-126 was significantly down-regulated(P< 0.05).HOTAIR targeted to inhibit the expression of miR-126, and miR-126 targeted to inhibit the expression of SHH.Compared with the sh-NC group, the cell viability, proliferation, migration and invasion of SW480 and HCT116 cells in sh-HOTAIR group decreased, while those in the miR-126 inhibitor group increased(P< 0.05).The cell viability, proliferation, migration and invasion of cells in the sh-HOTAIR+miR-126 inhibitor group were significantly higher than those in the sh-HOTAIR group, and significantly lower than those in the miR-126 inhibitor group(P< 0.05).The cell viability, proliferation, migration and invasion of cells in the miR-126 inhibitor+sh-SHH group were significantly lower than those in the miR-126 inhibitor group (P< 0.05).Conclusion:The expression of HOTAIR targets to inhibit the expression of miR-126, thereby promoting the occurrence and development of colorectal cancer.The mechanism may be related to the activation of SHH signaling pathway.