Effect and mechanism of circ_0006689 on IL2 in systemic lupus erythematosus based on Jurkat cells

Objective:To preliminarily explore the effect and mechanism of circ_0006689 in regulating interleukin 2(IL2)in systemic lupus erythematosus(SLE)on the basis of Jurkat cells.Methods:Jurkat cells were transfected with lentivirus to construct circ_0006689 low expression strain; the relative expression levels of IL2 and miR-95-5p were determined by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) after PMA and PHA activated the cells in low expression group (si-circ_0006689 group), virus-free group (si-NC group)and untransfected group(control group).The concentration of secreted IL2 in each group was detected by enzyme-linked immunosorbent assay (ELISA); bioinformatics analysis predicted the target miRNA of circ_0006689 and the target miRNA of IL2; dual-luciferase reporter assay was used to detect the targeting relationship between circ_0006689 and miR-95-5p, and miR-95-5p and IL2.Results:IL2 secretion significantly increased after Jurkat cell activation (P< 0.05); circ_0006689 was down-regulated, IL2 expression and secretion decreased(P< 0.05), and the expression level of miR-95-5p increased significantly (P< 0.05).The dual-luciferase reporter assay suggested the binding effect of circ_0006689 to miR-95-5p and miR-95-5p to IL2.Conclusion:Low expression of circ_0006689 in Jurkat cells can inhibit IL2 expression; miR-95-5p can bind to circ_0006689 and IL2, respectively; the circ_0006689 may affect IL2 expression and secretion by competitive binding to miR-95-5p in SLE.