Inhibitory effect of DMDD on hepatocellular carcinoma cells with insufficient radiofrequency ablation

Objective:To study the inhibitory effect of 2-dodecyl-6-methoxy-2, 5-diene-1, 4-cyclohexanedione(DMDD), isolated from the root of Averrhoa Carambola L., on the HuH-7 and Hep3B model of human hepatocel-lular carcinoma cells with insufficient radiofrequency ablation.Methods:An insufficient radiofrequency ab-lation cell model was constructed by treating human hepatocellular carcinoma cells HuH-7 and Hep3B with a 47℃water bath.Cell proliferation assay (CCK-8) was performed to detect the proliferation ability of wild-type HuH-7, Hep3B cells (control group) and insufficient ra-diofrequency ablation model cells(model group), the migration ability of cells was detected by scratch assay, and the invasion ability of cells was detected by cell invasion assay (Transwell).CCK-8 assay was used to detect the half-inhibitory concentration of DMDD on hepatocellular carcinoma cells HuH-7 and Hep3B with insufficient ra-diofrequency ablation.Three DMDD concentration gradients were selected: high-concentration DMDD group(10 μmol/L, DMDD H group), medium-concentration DMDD group (5 μmol/L, DMDD M group) and low-con-centration DMDD group (2.5 μmol/L DMDD L group).The proliferation, migration and invasion abilities of in-sufficient radiofrequency ablation cell model were detected after DMDD intervention.Western blotting assay was used to measure METTL3 protein expression levels in the insufficient radiofrequency ablation cell model before and after DMDD intervention.Results:Compared with the wild-type HuH-7 and Hep3B cells in the control group, the proliferation, migration and invasion abilities of hepatocellular carcinoma cells HuH-7, Hep3B with in-sufficient radiofrequency ablation were significantly enhanced (P< 0.05), and the expression level of METTL3 protein was up-regulated (P< 0.05).After DMDD intervention, the proliferation, migration, and invasion levels were significantly inhibited and METTL3 protein expression level was down-regulated in the DMDD H group compared with the control group (P< 0.05).Conclusion:A HuH-7 and Hep3B hepatocellular carcinoma cell model with insufficient radiofrequency ablation is successfully established, with strong proliferation, migration and invasion abilities.DMDD can inhibit the proliferation, migration, invasion and other malignant biological properties of hepatocellular carcinoma cells with insufficient radiofrequency ablation, and has anti-tumor effect.