Effect of hederagenin on growth, proliferation, migration and invasion of U87 MG cells

Objective:To explore the effect of hederagenin(Hed)on the growth, proliferation and motor capacity of glioblastoma cell line U87 MG.Methods:Hed was divided into 0 μmol/L group, 20 μmol/L group, 40 μmol/L group, 60 μmol/L group and 80 μmol/L group.The cell viability of Hed treated for 48 h was detected by CCK-8 method to determine the drug administration concentration.Cell growth was detected by plate cloning assay.DNA replication was detected by EdU staining.Cell cycle distribution was detected by flow cytometry.Cell migration and invasion were detected by wound healing assay and Transwell assay.The expressions of Cyclin D1, CDK2, CDK4 and MMP2 proteins in cells were detected by western blotting.Results:After U87 MG cells were treated with Hed for 48 h, U87 MG cells in 40 μmol/L group were significantly inhibited (P< 0.05).Compared with 0 μmol/L group, the clonal formation rate of U87 MG cells in 20 μmol/L group and 40 μmol/L group significantly decreased, the DNA replication was significantly inhibited, the proportion of G1 phase cells was significantly reduced, the wound healing rate significantly decreased, and the migration and invasion ability were significantly weakened (P< 0.05), and the inhibition was dose-dependent.The relative expressions of Cyclin D1, CDK2, CDK4 and MMP2 proteins were significantlyreduced (P< 0.05).Conclusion:Hed can inhibit the growth, proliferation and motor capacity of U87 MG glioblastoma, and its mechanism may be related to the inhibition of cell cycle related proteins and MMP2.