Preparation, mechanical properties and cytoactive of polycaprolactone/BaTiO₃ nanofiber membranes

Objective:To study the preparation, mechanical properties and cellular activities of polycaprolactone(PCL)/BaTiO₃ (BT) nanofiber membranes.Methods:PCL, PCL/3% BT, PCL/6% BT and PCL/9% BT nanofiber membranes were made by adding BaTiO₃ using electrospinning technology.Scanning electron microscopy(SEM)observed the diaphragm morphology and the growth morphology of mesenchymal stem cells(MSC), and mechanical properties tested the mechanical properties of materials.MSC proliferation on the membrane at 7 days was measured by CCK-8 assay.Cell viability was observed under SEM to explore the growth of bone marrow mesenchymal stem cells (BMSCs) on electrospun nanofiber membranes.Calcein-AM/PI staining was used to compare cell survival of MSCs in compound culture with nanofiber membranes for 7 d.Results:SEM results showed that the surface of pure PCL electrospinning film fiber was smooth with no depression or protrusion, PPCL/3%BT electrospinning film fiber surface had protrusion occasionally, PCL/6%BT electrospinning film fiber became sparse, and PCL/9%BT electrospinning film fiber surface was precipitated obviously.It was obvious that the porosity of PCL/6%BT was better than that of other groups.MSCs had significantly better cell proliferation and activity on PCL/6%BT membranesthan the other three membranes and a more dense cell distribution.Conclusion:In this study, PCL, PCL/3%BT, PCL/6%BT and PCL/9%BT nanofiber membranes are successfully prepared using electrospinning technology.The nanofiber membranes have good mechanical properties and have the ability to promote cell proliferation.