Xie Xuelu, Wu Pinghua. Effect of CK1ε on proliferation, migration and invasion of glioma cells and its prognosis[J]. Journal of Guangxi Medical University, 2023, 40(3): 351-356. DOI: 10.16190/j.cnki.45-1211/r.2023.03.002
Citation: Xie Xuelu, Wu Pinghua. Effect of CK1ε on proliferation, migration and invasion of glioma cells and its prognosis[J]. Journal of Guangxi Medical University, 2023, 40(3): 351-356. DOI: 10.16190/j.cnki.45-1211/r.2023.03.002

Effect of CK1ε on proliferation, migration and invasion of glioma cells and its prognosis

  • Objective: To explore the effect of casein kinase 1ε (CK1ε) on the proliferation, migration and invasion of glioma cells through Wnt/β-catenin pathway and the effect of CSNK1E (CK1ε coding gene) on the prognosis of patients with glioma.Methods: Human glioma U251 cells were cultured in vitro, and transfected with CK1ε overexpressing lentivirus vector or negative virus control, which were divided into blank control group, negative control group and experimental group.Cell counting kit(CCK-8)was used to detect U251 cell proliferation.Scratch test and Transwell test were used to detect U251 cell migration, and Transwell test was used to detect U251 cell invasion as well.Western blotting method was used to detect relative expression of β-catenin protein associated with Wnt/β-catenin pathway.Kaplan-Meier method was used to analyze the effect of CSNK1E expression in GEPIA database on the survival of patients with glioma; multivariate COX risk proportional regression model was used to analyze the effect of CSNK1E expression on the prognosis of patients with glioma in Chinese glioma genome map (CGGA).Results: The overexpression system of U251-CK1ε was established and CK1ε was successfully overexpressed in U251 cells; compared with blank control group and negative control group, the number of cell proliferation, migration and invasion in experimental group increased (all P<0.05), while the relative expression of β-catenin protein decreased (all P<0.05).The overall survival of patients with high CSNK1E expression was significantly longer than that with low expression (P<0.05).High grade gliomas, age higher than 50 years and IDH mutation were independent risk factors for survival of glioma patients (all P<0.05), while CSNK1E was not an independent factor for survival of glioma patients (P> 0.05).Conclusion: CK1ε can promote the proliferation, migration and invasion of U251 cells, which may be related to the inhibition of Wnt/β-catenin pathway; high malignancy, age higher than 50 years, and IDH mutation are associated with poor prognosis in glioma patients, while CSNK1E expression is not significantly correlated with poor prognosis of patients.
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