Mechanisms of honeysuckle in pelvic inflammatory disease based on network pharmacology

Objective: To explore the active components, targets and potential molecular mechanisms of honeysuckle in the treatment of pelvic inflammatory disease by network pharmacology.Methods: The active components and targets of honeysuckle were screened from TCMSP database, the targets of pelvic inflammatory disease were retrieved from GeneCards database, and the intersection of targets was normalized through Drugbank and Uniprot databases.Cytoscape 3.9.0 was used to construct drug-component-target network, and protein interaction network was constructed by intersection genes.Core target genes were analyzed by MCODE and Metascape database.GO bioenrichment analysis and KEGG pathway analysis were applied on related targets by Metascape database.The minimum inhibitory concentration of honeysuckle against Staphylococcus aureus was measured in vitro.Results: 119 effective targets of honeysuckle and 1038 disease targets related to pelvic inflammatory disease were screened, and 62 common targets were identified.The core genes in PPI network included APP, NTRK1, TP53, EGFR and ESR1.GO enrichment analysis mainly involved serine hydrolase activity, phosphatase binding, serine endopeptidase activity and protease binding.KEGG pathway enrichment analysis showed that the common targets played a role in multiple signaling pathways, such as atherosclerosis, AGE-RAGE and IL-17 through cellular fluid shear stress.In vitro experiments showed that honeysuckle had obvious inhibitory effect on Staphylococcus aureus at the concentration of 6.25 mg/mL or above.Conclusion: The targets and mechanisms of honeysuckle in the treatment of pelvic inflammatory disease have been predicted, and honeysuckle has inhibitory effect on Staphylococcus aureus.