Preparation and antibacterial properties of composite drug loading system of titanium alloy stent and gelatin

Objective: To improve the antibacterial properties and biocompatibility of titanium alloy implant materials.Methods: In this paper, a composite bone implant material was prepared by using 3D printed porous titanium alloy as a stent filled with gelatin containing gentamicin.The effects of different drug loadings on gelatin stability were evaluated by infrared absorption spectrum, water solubility and in vitro drug release experiments.The antibacterial properties of the composite were analyzed by bacterial adhesion, bacterial activity and inhibition zone experiments.The biocompatibility of the material was evaluated by cell viability experiments.Results: The water resistance of the different drug-loaded composite materials reached 6 days, and the drug release effect could be maintained until the gelatin was completely dissolved.The increase of drug loading had better antibacterial activity to E. coli and S. aureus, and the inhibition zone of the two bacteria was the largest at 8 mg/ml. Cytotoxicity analysis showed that the activity of osteoblasts was not significantly affected by different drug loading levels (P＞ 0.05).Conclusion: It is considered that the composite bone implant material has good antibacterial properties and biocompatibility in vitro.