Abstract: Endometriosis (EMs), characterized by the presence of endometrioid tissue outside the uterine cavity, is a common chronic benign recurrent disease in women of childbearing age, with a global incidence rate of about 10%. Due to its multifactorial nature and unclear pathogenesis, the current diagnostic gold standard for this disease is still laparoscopic pathology. There is a lack of sensitive and specific biomarkers in clinical practice. Finding accurate, reliable, and applicable non-invasive biomarkers is currently a hot topic and focus of research. This article summarizes the most important and novel research results of EMs-related glycoproteins, transcriptomics, and autoantibody biomarkers, and provides new strategies for early diagnosis and targeted therapy.