RAB11A异常高表达促进鼻咽癌侵袭能力

The abnormally high expression of RAB11A promotes the invasion of nasopharyngeal carcinoma

  • 摘要:
    目的 探索RAB11A在鼻咽癌(NPC)中的表达情况及其对NPC细胞恶性生物学行为的影响。
    方法 使用公共数据库GEO比较RAB11A在NPC组织和正常鼻咽上皮组织(NNE)中转录水平的差异,并通过western blotting以及免疫组织化学染色验证RAB11A蛋白表达水平。使用siRNA和过表达质粒分别在NPC细胞中敲低和过表达RAB11A。通过Transwell实验检测细胞的侵袭能力。
    结果 相较于NNE,RAB11A在NPC中呈高转录和高蛋白表达水平(均P<0.05)。受试者工作特征(ROC)曲线分析显示,RAB11A mRNA诊断NPC的曲线下面积(AUC)为0.748(95% CI:0.667~0.829),并且mRNA表达水平越高的患者生存时间越短(P=0.000 63)。此外,敲低RAB11A可以显著抑制NPC细胞的侵袭能力(P<0.05),反之过表达RAB11A则可以显著增强NPC细胞的侵袭能力(P<0.01)。
    结论 RAB11A是NPC潜在的肿瘤促进因子,可能为NPC的有效诊断和预后预测分子标志物。

     

    Abstract:
    Objective To investigate the expression of RAB11A in nasopharyngeal carcinoma (NPC) and its effect on the malignant biological behavior of NPC cells.
    Methods The differences in the transcription level of RAB11A in the NPC epithelium and the normal nasopharyngeal epithelium (NNE) were compared using a public database GEO, and protein expression level of RAB11A was verified by western blotting and immunohistochemistry techniques. RAB11A was knocked down and overexpressed in NPC cells using siRNA and overexpressed plasmid, respectively. The invasive ability of the cells was detected by Transwell assay.
    Results Compared with NNE, RAB11A showed higher transcription and protein expression levels in NPC (all P < 0.05). Receiver operating characteristic (ROC) curve analysis showed that the AUC of RAB11A mRNA in diagnosing NPC was 0.748 (95% CI: 0.667-0.829), and the survival time of patients with higher mRNA expression level was shorter (P= 0.00063). In addition, knockdown of RAB11A significantly inhibited the invasion ability of NPC cells (P < 0.05), whereas overexpression of RAB11A significantly enhanced the invasion ability of NPC cells (P < 0.01).
    Conclusion RAB11A is a potential tumor-promoting factor in NPC, and may be an effective molecular marker for diagnosis and prognosis prediction of NPC.

     

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