Abstract:
Objective To investigate the effect of lncRNA-Scarna10 (Scarna10) on resveratrol (RSV)-induced polarization of macrophages.
Methods M1 polarization model of RAW264.7 macrophages was established by LPS and treated with RSV for 24 hours. The study groups were divided into control group, LPS group and RSV+ LPS group. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of Scarna10, and western blotting and immunofluorescence were used to detect the expression of macrophage polarization markers (iNOS, Arg-1, CD206). RAW264.7 cells were divided into Si-NC group, LPS+Si-NC group, RSV+LPS+Si-NC group and RSV+LPS+Si-Scarna10 group after silencing Scarna10, and the expression of Scarna10 and macrophage polarization markers in each group after transfection was detected.
Results After LPS induction and resveratrol intervention, the protein expression level of iNOS, a M1 polarization marker, was elevated in the LPS group, the protein expression levels of Arg-1 and CD206, M2 polarization markers, were elevated, while the protein expression level of iNOS was decreased in the RSV+LPS group. At the same time, the expression level of Scarna10 in the RSV+LPS group was higher than that in the LPS group. After silencing Scarna10, compared with the RSV+LPS+Si-NC group, the expression level of Scarna10 and the protein expression levels of Arg-1 and CD206 in RSV+LPS+Si-Scarna10 group were decreased, and the protein expression level of iNOS was elevated. The differences among the above groups were statistically significant (all P < 0.05).
Conclusion The expression of Scarna10 is upregulated in M1 macrophages following RSV intervention, and silencing Scarna10 can reverse the RSV-induced promotion of M1-to-M2 macrophage polarization.
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