Abstract: Objective: To prepare the standard decoction of Vernonia patula and establish the quality standard of Vernonia patula standard decoction, so as to provide reference for formulation of the quality standard of Vernonia patula formula granules and its related preparations. Methods: Fifteen batches of Vernonia patula standard decoction were prepared. The fingerprints of decoction pieces and standard decoction were established, and the common peaks in the fingerprints were confirmed. The law of quality value transmitting of Vernonia patula standard decoction was analyzed by the transfer number of common peaks, the transfer rate and extraction ratio of the index components. Results: There were 7 common peaks in the fingerprints of Vernonia patula decoction pieces and standard decoction, indicating that the common peaks could be transferred from the decoction pieces to the standard decoction. The similarity of fingerprints of Vernonia patula and standard decoction was greater than 0.9. The content range of chlorogenic acid in 15 batches of standard decoction was 0.25-0.40 mg/g, with an average of 0.34 mg/g, and the transfer rate range was 26.19%-38.52%. The content range of caffeic acid was 0.07-0.12 mg/g and the transfer rate range was 84.37%-134.93%. The extraction rate of 15 batches of Vernonia patula standard decoction was between 11.53% and 14.02%, and the extracts ranged from 6.42% to 9.16%. Conclusion: The fingerprints and content determination method of high-performance liquid chromatography (HPLC) method demonstrate good precision, repeatability, and stability, which provides reference for the formulation of the quality standards of Vernonia patula and preparations. Additionally, it lays a foundation for the quality control of Vernonia patula traditional Chinese medicine formula granule products.