Mechanism of RASA4 expression inactivation promoting proliferation of nasopharyngeal carcinoma
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摘要 目的：探究RASA4 基因在鼻咽癌（NPC）中的表达情况，研究RASA4 基因对影响NPC 增殖和转移的影响及分子机制。方法：利用GEO数据库进行NPC中RASA4转录水平的Meta分析。采用实时荧光定量PCR（RT-qPCR）和western blotting法检测NPC 细胞中RASA4 mRNA 及蛋白表达水平，免疫组织化学染色法检测RASA4 在NPC 组织中的蛋白表达及定位情况，CCK-8 及平板克隆实验评估RASA4 对NPC 细胞增殖能力的影响。结果：基于GEO 数据的生物信息学分析显示，RASA4 在NPC 中的mRNA 表达显著下调。RT-qPCR 和western blotting 显示NPC 细胞中RASA4 mRNA 及蛋白表达下调。外源性过表达RASA4基因可抑制NPC细胞增殖。结论：RASA4可能是NPC潜在的抑制基因，研究其失活机制及其功能对于NPC的发生机制和治疗具有重要意义。Abstract Objective: To explore the expression of RASA4 gene in nasopharyngeal carcinoma (NPC), and to investigate the influence and molecular mechanism of RASA4 gene affecting the proliferation of NPC. Methods: Meta-analysis of RASA4 transcription levels in NPC was performed using GEO database. RASA4 mRNA and protein expression levels in NPC cell lines were detected by reverse transcription-quantitative PCR (RT-qPCR) and western blotting. RASA4 protein expression and localization in NPC tissues were detected using immunohistochemistry (IHC). The impact of RASA4 on cell proliferation was evaluated through cell counting kit-8 (CCK-8) assay and colony formation assay. Results: Bioinformatics analysis based on GEO data showed that RASA4 mRNA expression was significantly down-regulated in NPC. RT-qPCR and western blotting results indicated that RASA4 was down-regulated at both mRNA and protein expression in NPC cells. Exogenous overexpression of the RASA4 gene could inhibit the cell proliferation of NPC. Conclusion: RASA4 may serve as a potential suppressor gene in NPC, and investigating its inactivation mechanism and function is of significant importance for the pathogenesis and treatment of NPC.
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Keywords
- nasopharyngeal carcinoma /
- RASA4 /
- cell proliferation
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