Abstract: Objective: To explore the expression of RASA4 gene in nasopharyngeal carcinoma (NPC), and to investigate the influence and molecular mechanism of RASA4 gene affecting the proliferation of NPC. Methods: Meta-analysis of RASA4 transcription levels in NPC was performed using GEO database. RASA4 mRNA and protein expression levels in NPC cell lines were detected by reverse transcription-quantitative PCR (RT-qPCR) and western blotting. RASA4 protein expression and localization in NPC tissues were detected using immunohistochemistry (IHC). The impact of RASA4 on cell proliferation was evaluated through cell counting kit-8 (CCK-8) assay and colony formation assay. Results: Bioinformatics analysis based on GEO data showed that RASA4 mRNA expression was significantly down-regulated in NPC. RT-qPCR and western blotting results indicated that RASA4 was down-regulated at both mRNA and protein expression in NPC cells. Exogenous overexpression of the RASA4 gene could inhibit the cell proliferation of NPC. Conclusion: RASA4 may serve as a potential suppressor gene in NPC, and investigating its inactivation mechanism and function is of significant importance for the pathogenesis and treatment of NPC.