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    基于肠道菌群探讨运动对2型糖尿病小鼠血糖的影响

    Effect of exercise on blood glucose in type 2 diabetes mellitus mice based on gut microbiota

      摘要
    • 摘要: 目的：基于肠道菌群探讨运动对2型糖尿病（T2DM）小鼠血糖、体重及其胰高血糖素样肽1（GLP-1）的影响。方法：采用6 只8 周龄雄性C57BKS 小鼠作为野生型对照组（DZ 组）和18 只8 周龄雄性db/db 小鼠作为T2DM 小鼠模型，将18 只T2DM 小鼠随机分为T2DM 模型+联合运动组（F 组）、T2DM 模型+灌肠组（SY 组）、T2DM 模型组（TJ 组），每组6 只。F 组进行8 周有氧联合抗阻运动干预；SY 组采用F 组运动8周后的小鼠粪便制成悬浊液灌肠，2次/d，共14 d；TJ组采用DZ 组小鼠粪便制成悬浊液灌肠，2 次/d，共14 d；DZ 组不做干预。灌肠结束后，检测各组小鼠粪便肠道菌群、血糖、体重及血清GLP-1 含量。结果：SY组小鼠肠道菌群的chao 1指数和shannon指数显著高于DZ组和TJ组（P< 0.05）；在门水平上，与DZ组比较，SY组小鼠的肠道厚壁菌门的相对丰度均上升（P< 0.05），变形菌门和拟杆菌门的相对丰度比例下降（P< 0.05）；属水平上，与DZ 组比较，SY 组提高了毛螺菌属（Lachnospiraceae_）的相对丰度（P< 0.05）。与TJ组比较，SY组小鼠肠道厚壁菌门细菌比例上升，变形菌门比例下降（均P< 0.05）。SY 组小鼠的GLP-1 水平高于DZ 组和TJ 组，血糖和体重水平低于DZ 组和TJ 组，差异均有统计学意义（P< 0.05）。结论：联合有氧—抗阻运动可改善肠道有益菌的丰度和结构，提高T2DM 小鼠肠道产短链脂肪酸的菌群及血清GLP-1水平，降低小鼠血糖水平和体重，从而改善T2DM。

       

      Abstract: Objective: To investigate the effect of exercise on blood glucose, body weight and glucagon-like pep-tide-1(GLP-1)in type 2 diabetes mellitus(T2DM)mice based on gut microbiota.Methods: Six 8-week-old male C57BKS mice were used as wild-type control group (DZ), and eighteen 8-week-old male db/db mice were used as T2DM mouse models and randomly divided into T2DM model+combined exercise group (F group), T2DM model+enema group (SY group), and T2DM model group (TJ group), with 6 mice in each group.The F group underwent aerobic and resistance exercise for 8 weeks.In the SY group, the feces of mice in the F group after 8 weeks of exercise were made into suspension enema, twice a day for 14 days.In the TJ group, the feces suspen-sion made from DZ mice was given enema twice a day for 14 days.No intervention was performed in the DZ group.After the end of enema, the fecal gut microbiota, blood glucose, body weight and serum GLP-1 content were detected.Results: The chao 1 index and shannon index of gut microbiota in the SY group were significantly higher than those in the DZ and TJ groups(P< 0.05); at the phylum level, compared with the DZ group, the rela-tive abundance of Firmicutes in the SY group increased (P< 0.05), while the relative abundance ratios of Proteo-bacteria and Bacteroidetes decreased(P< 0.05); at the genus level, compared with the DZ group, the SY group in-creased the relative abundance of Lachnospiraceae(P< 0.05).Compared with the TJ group, the proportion of Fir-micutes increased, and the proportion of Proteobacteria decreased in the SY group (both P< 0.05).The level of GLP-1 in the SY group was higher than that in the DZ and TJ groups, and the level of blood glucose and body weight were lower than those in the DZ and TJ groups, and the differences were statistically signifi-cant (P< 0.05).Conclusion: Combined aerobic-resistance exercise can improve the abundance and structure of beneficial bacteria in gut microbiota, increase the intestinal short-chain fatty acid producing bacteria and serum GLP-1 level, and reduce blood glucose and body weight in T2DM mice, thereby improving T2DM.

       

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