Abstract: Objective: To investigate the effect of forkhead box M1 (FoxM1) on proliferation, invasion, and apoptosis of cisplatin resistant human cervical cancer strain Hela/DDP and its mechanism.Methods: Hela/DDP cells were divided into blank group (conventional culture without any treatment), normal control (NC) group (transfection with blank plasmid), low-expression group, and overexpression group.The low-expression group transfected with siRNA-FoxM1 to downregulate the expression of FoxM1 in Hela/DDP cells, while the overexpression group cells transfected with pcDNA3.1-FoxM1 to upregulate the expression of FoxM1 in Hela/DDP cells.The expression level of FoxM1 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR).The cell viability was detected by CCK-8.Apoptosis was detected by flow cytometry, and invasive ability of cells was detected by Transwell.Results: The expression level of FoxM1 mRNA in Hela cells was lower than that in Hela/DDP cells (P< 0.05).There was no significant difference in FoxM1 mRNA, proliferation rate, apoptosis rate, the number of invasive cells, and the expression levels of HSP70 and S100A9 proteins between the blank group and the NC group (P> 0.05).Compared with the blank group, the cell viability and invasive ability as well as the expression levels of FoxM1 mRNA and HSP70 protein in the low-expression group decreased, while the apoptosis rate and S100A9 protein expression level increased (P< 0.05).Compared with the blank group, the cell viability and invasive ability as well as the expression levels of FoxM1 mRNA and HSP70 protein in the overexpression group increased, while the apoptosis rate and S100A9 protein expression level decreased (P< 0.05).Compared with the low-expression group, the cell viability, invasion ability, and expression levels of FoxM1 mRNA and HSP70 protein elevated in the overexpression group, while the apoptosis rate and S100A9 protein expression level reduced (P< 0.05).Conclusion: Down-regulating the mRNA level of FoxM1 can inhibit the expression of HSP70, promote the expression of S100A9, and regulate the malignant behavior of cervical cancer cells, thereby improving the sensitivity of cervical cancer cell lines resistant to cisplatin chemotherapy.