Abstract: Objective:To study the effect of Lactobacillus crimpus on the migration and invasion ability of cervical cancer cells and its mechanism.Methods: Lactobacillus crimpus was co-cultured with SiHa cells of cervical squamous cell carcinoma under suitable conditions.Transwell test was used to observe the changes of cell migration and invasion ability before and after co-culture.Meanwhile, real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and western blotting were used to detect the gene and protein expressions of ABCG2, PCNA, ATM, TDG, LIG1, OGG1 and HMGB1.Results: Lactobacillus crimpus could effectively inhibit the migration and invasion of SiHa cells after co-culture for 18 h and 24 h(P< 0.001).At the same time, the oncogenes PCNA, ATM and LIG1 associated with cervical cancer began to decrease significantly after 12 h co-culture (P< 0.05); OGG1 and HMGB1 decreased significantly after co-culture for 18 h (P< 0.05); no significant changes were observed between ABCG2 and TDG before and after co-culture (P> 0.05).In addition, the expressions of PCNA, OGG1 and HMGB1 proteins began to decrease significantly after 12 h co-culture (P< 0.05), ABCG2, LIG1 and ATM proteins began to decrease significantly after 18 h co-culture(P< 0.05), and there were no significant changes in the relative protein expression levels before and after TDG co-culture (P> 0.05).Conclusion:Lactobacillus crimpus can effectively inhibit the migration and invasion of SiHa cells of cervical cancer and inhibit the expressions of PCNA, ATM, LIG1, OGG1, HMGB1 and other oncogenes.