Abstract: Objective:To explore the anti-inflammatory and anti-oxidant effect of the Dalbergia odorifera T.Chen extract(DOE)on lipopolysaccharide (LPS)-induced RAW264.7 cells and the hair growth-promoting effect on human dermal papilla cells(hDPCs).Methods:RAW264.7 cells were divided into blank control group, model group, 4% DOE group and 8% DOE group.The blank control group was untreated, the model group was treated with LPS to induce the inflammatory model in vitro, and the 4% DOE and 8% DOE groups were treated with LPS and then treated with 4% DOE and 8% DOE, respectively.The contents of nitric oxide (NO) and tumor ne-crosis factor-α(TNF-α)in the supernatants of cell culture in each group were determined by Griess assay and en-zyme-linked immunosorbent assay (ELISA), respectively.The contents of intracellular reactive oxygen species(ROS) in each group were detected by DCFH-DA fluorescent probe.HDPCs isolated from human hair follicles were cultured in vitro, and were divided into the blank control group, the 4% DOE and 8% DOE groups.The via-bility of hDPCs was detected by CCK-8 assay.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was performed to detect the expressions of hair-growth factor CTNNB1 and hair inhibition factor DKK1.Western blotting was performed to de-tect the expression of β-catenin protein.Results:Compared with the model group, the NO, TNF-α, and ROS contents in RAW264.7 cells in the 4% and 8% DOE groups decreased(all P< 0.05).Compared with the blank control group, hDPCs cell viability increased, CTNNB1 gene and β-catenin protein expression were up-regulated, and DKK1 gene expression was down-regulated in the 4% and 8% DOE groups (all P< 0.05).Conclusion:DOE has anti-inflammatory and anti-oxidant effect on LPSinduced RAW264.7 macrophages, and it can promote the hair growth by increasing the viability of hDPCs and regulating the expression of key genes.