高级检索

    负载葛根素的聚己内酯/明胶纳米纤维膜诱导成骨和抗菌的研究

    Study on induced osteogenesis and antibacterial activity of polycaprolactone/gelatin nanofiber membrane loaded with puerarin

      摘要
    • 摘要: 目的：用静电纺丝技术将聚己内酯（PCL）、明胶（GEL）、葛根素（PUE）制备成PCL/GEL@PUE纳米纤维膜，验证其生物相容性、机械性能、成骨及抗菌性能。方法：在电纺前溶液中分别加入0% （w/v）、0.1% （w/v）、0.2% （w/v）的PUE，制成PCL/GEL、PCL/GEL@0.1% PUE、PCL/GEL@0.2% PUE 3组纳米纤维膜。利用扫描电子显微镜（SEM）观察纳米纤维膜的结构；傅里叶红外光谱分析仪（FTIR）确认制备成功；力学性能测试纳米纤维膜机械强度。将骨髓间充质干细胞接种于各纳米纤维膜上，通过细胞计数试剂（CCK-8）法、钙黄绿素/碘化丙啶（Calcein-AM/PI）染色检测细胞相容性；茜素红染色检测成骨性能。将耐甲氧西林金黄色葡萄球菌与各组纳米纤维膜共培养，并涂琼脂板，计数菌落，检测抑菌率。结果：SEM结果显示，加入PUE后纤维变细，孔径变小，表面变粗糙；FTIR结果显示PCL/GEL@PUE纳米纤维膜构建成功；力学测试结果表明，加入PUE后，纳米纤维膜机械性能增强。CCK-8及Calcein-AM/PI 染色结果显示，各组纳米纤维膜在第7天时能明显促进骨髓间充质干细胞增殖，其中PCL/GEL@0.2% PUE 组细胞相容性最好。茜素红染色结果表明，PCL/GEL 纳米纤维膜无明显成骨作用，而PCL/GEL@0.1% PUE、PCL/GEL@0.2% PUE 纳米纤维膜成骨作用递增。抗菌实验结果表明，PCL/GEL 纳米纤维膜无抗菌作用，而PCL/GEL@0.1% PUE、PCL/GEL@0.2% PUE 纳米纤维膜分别有48% 、86% 的抑菌率。结论：负载PUE0.1% （w/v）、0.2% （w/v）的PCL/GEL纳米纤维膜具有良好的成骨性能和抗菌性能，其中以PCL/GEL@0.2% PUE组效果最好。

       

      Abstract: Objective:To prepare PCL/GEL@PUE nanofiber membrane with polycaprolactone (PCL), gelatin(GEL), and puerarin (PUE) by electrospinning technology, and to verify its biocompatibility, mechanical properties, osteogenesis and antibacterial properties.Methods:PCL/GEL, PCL/GEL@0.1% PUE and PCL/GEL@0.2% PUE nanofiber membranes were prepared by adding 0% (w/v), 0.1% (w/v), and 0.2% (w/v)of PUE into the preelectrospinning solution.The structures of nanofiber membranes were observed by scanning electron microscope(SEM); the preparation was confirmed by Fourier infrared spectroscopy (FTIR); mechanical properties tested the mechanical strength of nanofiber membranes.The bone marrow mesenchymal stem cells were inoculated on the nanofiber membranes and the cell compatibility was tested by CCK-8 test and Calcein-AM/PI staining; alizarin red S staining was used to detect the osteogenic properties.Methicillin-resistant Staph-ylococcus aureus was co-cultured with nanofiber membranes of each group and coated with agar plates to count the colonies and test the bacteriostasis rate.Results:The results of SEM showed that the fibers became thinner, the pore diameter became smaller, and the surface became rougher after adding PUE; FTIR results showed that PCL/GEL@PUE nanofiber membrane was successfully constructed; the mechanical test results showed that the mechanical properties of nanofiber membranes were enhanced after adding PUE.The results of CCK-8 and Calcein-AM/PI staining showed that the nanofiber membranes in each group could significantly promote the proliferation of bone marrow mesenchymal stem cells on the 7th day, and the PCL/GEL@0.2% PUE group had the best cytocompatibility.Alizarin red S staining showed that polycaprolactone/gelatin nanofiber membrane had no obvious osteogenic effect, while PCL/GEL@0.1% PUE and PCL/GEL@0.2% PUE nanofiber membranes had increasing osteogenic effect.The results of the antibacterial experiment showed that PCL/GEL nanofiber membrane had no antibacterial effect, while PCL/GEL@0.1% PUE and PCL/GEL@0.2% PUE nanofiber membranes had 48% and 86% antibacterial rates, respectively.Conclusion:PCL/GEL nanofiber membranes loaded with PUE 0.1% (w/v), 0.2% (w/v)show good osteogenic and antibacterial properties, and PCL/GEL@0.2% PUE group shows the best effect.

       

      • HTML全文
      • 参考文献(0)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回