Abstract: Objective: To explore the effect of G-protein coupled receptor 1 (GPR1) on the proliferation, migration and invasion of human hepatocellular carcinoma(HCC)cells.Methods: Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of GPR1 mRNA in human hepatocellular carcinoma cell lines (Huh7, QGY-7703, HCCLM3, Hep3B, HepG2) and human immortalized liver cell line(THLE2).Western blotting was used to verify the protein expression after knockdown of GPR1.The knockdown of GPR1 in Huh7 and QGY-7703 cell lines were achieved via targeting short hairpin RNA(shRNA).CCK-8 assay was used to analyze the proliferation ability of HCC cells after GPR1 knockdown.Scratch assay was used to analyze the migration ability of HCC cells after GPR1 knockdown, and transwell assay was used to analyze the invasion ability of HCC cells after GPR1 knockdown.Results: GPR1 was highly expressed in HCC Huh7 cells and QGY-7703 cells (P＜0.01).After knockdown of GPR1, the proliferation, migration and invasion abilities of HCC cells in the shGPR1 group were weakened compared with shNC group (P＜0.01).Conclusion: GPR1 is a cancer-promoting factor, and knockdown of GPR1 may inhibit the proliferation, migration and invasion of human HCC cells.