Abstract:
Objective: To investigate the expressions of toll-like receptor 2 (
TLR2), NK cell surface perforin(PRF)and granulozyme B(GrzB)in hemophagocytic syndrome in children and their relationship with prognosis.
Methods: Seventy-nine children with hemophagocytic syndrome admitted to Kunming Children's Hospital from January 2019 to January 2021 were selected as the observation group, and another 79 healthy children of the same age with physical examination were included as the control group.
TLR2 gene expression in peripheral blood was determined by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and the rate of PRF and GrzB positive cells on the surface of NK cells was determined by flow cytometry.The diagnostic effi-cacy of
TLR2, PRF and GrzB was analyzed by receiver operating characteristic curve (ROC).The observation group was followed up for 6 months, and patients' peripheral blood
TLR2 levels and the rate of PRF and GrzB positive cells on the surface of NK cells were compared between the death group and the survival group.Logistic regression was used to analyse the effect of the interaction of
TLR2, PRF and GrzB on the prognosis of haemo-phagocytic syndrome in children.
Results: In the observation group, the peripheral blood
TLR2 level was higher than that in the control group, and the rate of NK cell surface PRF and GrzB positive cells was lower than that in the control group (
P< 0.05); the expression level of
TLR2 in peripheral blood and PRF and GrzB on the surface of NK cells combined in the diagnosis of hemophago-cytic syndrome in children was significantly better than the single diagnosis; the expression level of
TLR2 in peripheral blood of the death group was lower than that in the survival group, and the rate of PRF and GrzB positive cells on the NK cell surface was higher than that in the survival group (
P< 0.05); there was an interac-tion between low TLR2 and high NK cell surface PRF and GrzB (
P< 0.05).
Conclusion: The combined detec-tion of
TLR2 expression in peripheral blood and the rate of PRF and GrzB positive cells on the surface of NK cells can improve the diagnostic efficiency of hemophagocytic syndrome in children, and interact with the prog-nosis of children, which is conducive to early detection and treatment.