Abstract: Objective: To analyze the expression and significance of Kinesin family member 2C(KIF2C) in hepatocellular carcinoma (HCC), and to explore the potential role of competitive endogenous RNA(ceRNA) network in the development of HCC.Methods: Firstly, the tumor on-chip database (Oncomine), The Cancer Genome Atlas(TCGA) and gene expression profile interaction analysis (GEPIA) databases were used to analyze the expression of KIF2C in HCC and its relationship with clinical parameters such as overall sur-vival (OS), disease-free survival (DFS) and pathological grading of HCC.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to analyze the expression of KIF2C in HCC tissues and matched paracancerous tissues.Secondly, the effect of interfering with KIF2C expression on HCC cell proliferation, inva-sion and migration was analyzed by cell function experiments.Then, the proteins and genes interacting with KIF2C were explored using STRING and GeneMANIA database.High-throughput sequencing was used to search for differential genes under the condition of log₂|FC|> 1, and enrichment analysis of GO and KEGG was carried out to explore the potential pathway of KIF2C.Finally, the interacting RNAs were screened and the ceR-NA regulatory network was constructed through co-expression analysis.Results: KIF2C was highly expressed in HCC (P< 0.001); KIF2C expression level affected the survival, prognosis and pathological grade of HCC pa-tients (P< 0.05); cell function experiments showed that overexpression of KIF2C promoted the proliferation, in-vasion and migration of HCC cells(P< 0.05);there were 10 proteins involved in KIF2C protein interaction; there were 20 genes involved in gene interaction of KIF2C; 3, 754 differentially expressed long non-coding RNA(DEln-cRNA), 7, 621 differentially expressed messengers RNA (DEmRNA) and 170 differentially expressed micro-RNA (DEmiRNA) were screened; GO and KEGG enrichment analysis showed that related differential genes were involved in functions such as extracellular vesicles and intrinsic components of plasma membrane, and were significantly enriched in p53 signaling pathway, PI3K-Akt signaling pathway and MAPK signaling pathway; fi-nally, 12 lncrnas and 5 mirnas formed a ceRNA network, among which has-miR-181b-5p was the key miRNA.Fi-nally, 12 lncRNAs and 5 miRNAs formed a ceRNA network, among which has-miR-181b-5p was the key miR-NA.Conclusion: KIF2C is highly expressed in HCC and suggests a poor prognosis.Overexpression of KIF2C promotes the proliferation, invasion and migration of HCC cells.The ceRNA regulatory network associated with KIF2C is obtained, which plays an important role in HCC.